LOCUS       sequence-132371-        5600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vectors for the salycilate cascade expression system 
            (Pnah/NahR-Psal/XylS2).ApR, expression vector with 
            rrnBT1T2-Pm-MCSII, M13 replication origin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Medina C, Camacho EM, Flores A, Mesa-Pereira B, Santero E
  TITLE     Improved expression systems for regulated expression in Salmonella 
            infecting eukaryotic cells.
  JOURNAL   PLoS One. 2011;6(8):e23055. doi: 10.1371/journal.pone.0023055. Epub 
            2011 Aug 1.
  PUBMED    21829692
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(8):e23055. doi"; pages: " 
            10.1371/journal.pone.0023055. Epub 2011 Aug 1"
COMMENT     Sequence Label: pMPO 54
FEATURES             Location/Qualifiers
     source          1..5600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      409..495
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        590..670
                     /label=Pm promoter
                     /note="The bacterial Pm promoter is activated by XylS in 
                     the presence of benzoate or m-toluate (Marques et al., 
                     1999)."
     primer_bind     complement(1061..1080)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1062..1080)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1090..1107)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1090..1106)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1099..1121)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1248..1703
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1335..1354)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1545..1566
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1729..1833
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1834..1902,1903..2694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2052..2071)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(3717..4667)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      4715..4937
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
ORIGIN
        1 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
       61 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
      121 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
      181 cgccgaaaat gacccagagc gctgccggca ccctgtccta cgagttgcat gataaagaag
      241 acagtcataa gtgcggcgac gaccaccgcg gtggcggccg cgcgaatttg cctggcggca
      301 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
      361 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
      421 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
      481 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
      541 tggcgggcag gacgcccgcc ataaactgcc agggaatttg atagagataa gtccagcctt
      601 gcaagaagcg gatacaggag tgcaaaaaat ggctatctct agaaaggcct accccttagg
      661 ctttatgcaa cagaaacaat aataatggag tcatgaccat gacaatgcac ctggggctcg
      721 actatataga tagtctcgtt gaagaagatg agaacgaggg catctaccgc tgcaagcgcg
      781 agatgttcac cgaccctcgg ctgttcgatt tagagatgaa acacatcttt gagggcaact
      841 ggatttatct cgcccacgag agccagattc ccgagaagaa cgactattac accacgcaga
      901 tgggccggca gccgatattc atcacacgca acaaagatgg tgagctgaat gccttcgtca
      961 atgcctgaat tcccaattcg gttcacccgg gcatatggca agaactgcag atatccatgg
     1021 taccgagctc ggatccgtcg acaagcttat cgccaattcg ccctatagtg agtcgtatta
     1081 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     1141 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     1201 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     1261 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     1321 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     1381 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     1441 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     1501 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     1561 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     1621 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     1681 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     1741 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1801 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1861 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1921 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1981 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     2041 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     2101 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     2161 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     2221 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     2281 ttgcacaaca tgggggatca tgtaactcgc cttgatcgct gggaaccgga gctgaatgaa
     2341 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     2401 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     2461 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     2521 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2581 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2641 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2701 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2761 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2821 ttccactgag cgtcagatct ctgttcgggg gttcactgac gctgcgtgac tacgatggtc
     2881 aggttgcgga ggctatggcc ctggtacgag cgctgaacaa aatgacgaaa gcaggtatgc
     2941 ctgaaagcgt gcgtattgcc tgaaaacaca acccgctacg ggggagactt acccgaaatc
     3001 tgatttattc aacaaagcca ctttcagtat ggatttgggt gttacggggg ggggttggtg
     3061 cttaaaatcg cgctgaggat ttctgatggt gttaagcggg cggttttgag atgtaaactc
     3121 gcccgtttaa cataatggat cttgcgcgca ccgcccgaac accactcgcc acaaaaaacc
     3181 gccggaacgt ccaaaagtac gggttttgct gcccgcaaac gggctgttct ggtgttgcta
     3241 gtttgttatc agaatcgcag atccggcttc agccggtttg ccggctgaaa gcgctatttc
     3301 ttccagaatt gccatgattt tttccccacg ggaggcgtca ctggctcccg tgttgtcggc
     3361 agctttgatt cgataagcag catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct
     3421 gtaacaagtt gtctcaggtg ttcaatttca tgttctagtt gctttgtttt actggtttca
     3481 cctgttctat taggtgttac atgctgttca tctgttacat tgtcgatctg ttcatggtga
     3541 acagctttga atgcaccaaa aactcgtaaa agctctgatg tatctatctt ttttacaccg
     3601 ttttcatctg tgcatatatg gacagttttc cctttgatat gtaacggtga acagttgttc
     3661 tacttttgtt tgttagtctt gatgcttcac tgatagatac aagagccata agaacctcag
     3721 atccttccgt atttagccag tatgttctct agtgtggttc gttgtttttg cgtgagccat
     3781 gagaacgaac cattgagatc atacttactt tgcatgtcac tcaaaaattt tgcctcaaaa
     3841 ctggtgagct gaatttttgc agttaaagca tcgtgtagtg tttttcttag tccgttatgt
     3901 aggtaggaat ctgatgtaat ggttgttggt attttgtcac cattcatttt tatctggttg
     3961 ttctcaagtt cggttacgag atccatttgt ctatctagtt caacttggaa aatcaacgta
     4021 tcagtcgggc ggcctcgctt atcaaccacc aatttcatat tgctgtaagt gtttaaatct
     4081 ttacttattg gtttcaaaac ccattggtta agccttttaa actcatggta gttattttca
     4141 agcattaaca tgaacttaaa ttcatcaagg ctaatctcta tatttgcctt gtgagttttc
     4201 ttttgtgtta gttcttttaa taaccactca taaatcctca tagagtattt gttttcaaaa
     4261 gacttaacat gttccagatt atattttatg aattttttta actggaaaag ataaggcaat
     4321 atctcttcac taaaaactaa ttctaatttt tcgcttgaga acttggcata gtttgtccac
     4381 tggaaaatct caaagccttt aaccaaagga ttcctgattt ccacagttct cgtcatcagc
     4441 tctctggttg ctttagctaa tacaccataa gcattttccc tactgatgtt catcatctga
     4501 gcgtattggt tataagtgaa cgataccgtc cgttctttcc ttgtagggtt ttcaatcgtg
     4561 gggttgagta gtgccacaca gcataaaatt agcttggttt catgctccgt taagtcatag
     4621 cgactaatcg ctagttcatt tgctttgaaa acaactaatt cagacataca tctcaattgg
     4681 tctaggtgat tttaatcact ataccaattg agatgggcta gtcaatgata attactagtc
     4741 cttttccttt gagttgtggg tatctgtaaa ttctgctaga cctttgctgg aaaacttgta
     4801 aattctgcta gaccctctgt aaattccgct agacctttgt gtgttttttt tgtttatatt
     4861 caagtggtta taatttatag aataaagaaa gaataaaaaa agataaaaag aatagatccc
     4921 agccctgtgt ataactcact actttagtca gttccgcagt attacaaaag gatgtcgcaa
     4981 acgctgtttg ctcctctaca aaacagacct taaaacccta aaggcttaag tagcaccctc
     5041 gcaagctcgg gcaaatcgct gaatattcct tttgtctccg accatcaggc acctgagtcg
     5101 ctgtcttttt cgtgacattc agttcgctgc gctcacggct ctggcagtga atgggggtaa
     5161 atggcactac aggcgccttt tatggattca tgcaaggaaa ctacccataa tacaagaaaa
     5221 gcccgtcacg ggcttctcag ggcgttttat ggcgggtctg cggagtggtg aatccgttag
     5281 cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa
     5341 cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt
     5401 gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccaatgatcg aagttaggct
     5461 ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga
     5521 cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg
     5581 gaaggccatc cagcctcgcg
//
