LOCUS       sequence-132375-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression of EGFP-OMP25C. For visualization of neuronal 
            mitochondria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Kysenius K, Brunello CA, Huttunen HJ
  TITLE     Mitochondria and NMDA receptor-dependent toxicity of berberine 
            sensitizes neurons to glutamate and rotenone injury.
  JOURNAL   PLoS One. 2014 Sep 5;9(9):e107129. doi: 
            10.1371/journal.pone.0107129. eCollection 2014.
  PUBMED    25192195
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-09-5"; volume: "9(9):e107129. doi"; pages: " 
            10.1371/journal.pone.0107129. eCollection 2014"
COMMENT     Sequence Label: pLenSyn2-EGFP-­OMP25C
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      10..19
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    20..76
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 ccttgctcac catggtggcg gatcccgggc ccgcggtacc gtcgactgca gaattcgaag
       61 cttgagctcg atatctgagt ccggtcgagt tggcgcgcct agttaattaa tgcggccgca
      121 ccggtagctc tagaggttta aacagctagc ccaaggggca gtgggtctgt gggccgcggg
      181 ggcggagcgc gaggggctca ggagcacggc ggtgctctct ggcacgacgc gactcctctg
      241 ctgcccgctg cagactgagg cagcgccgag gctctgccgc cgcagagcgt atggtcgcgc
      301 ccgcacctcc ctatcttgca cctctcgtgg tgcggtccga ctggactcac ggcagcatgg
      361 acgcgaccaa ggcggcgagg aaggggagtt tggggagggc tagtgagtgg cgtcagtcag
      421 cctttggtgc tggggaggtg gtggtgcgcg ccggcagcgg aggcgaaggc tctgaccgcg
      481 gtgctgaatc cgacagtgcg catgcgccgc gccgcaacct cttcccctcc ccctctgatg
      541 ggggcggggg atgcgcaatt tggggagtgg gggtggggtt cttgtcctga gggtccggac
      601 ctgtagtaac gaaactccct cccacgatcc taccctgttt accaacctct tcctattcct
      661 taggctaact tgcacttaca cactcttgcc cttcccagat acttgggttt ccagtgttga
      721 ttttcacctt tgaaggcatt ttggggatag aagcacactc agaggggaac ataaatgtag
      781 aaattctcct tcctgcagtg ttcc
//
