LOCUS       sequence-132379-         439 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation transfer plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 439)
  AUTHORS   Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, Hinkle G, 
            Piqani B, Eisenhaure TM, Luo B, Grenier JK, Carpenter AE, Foo SY, 
            Stewart SA, Stockwell BR, Hacohen N, Hahn WC, Lander ES, Sabatini 
            DM, Root DE
  TITLE     A lentiviral RNAi library for human and mouse genes applied to an 
            arrayed viral high-content screen.
  JOURNAL   Cell. 2006 Mar 24. 124(6):1283-98.
  PUBMED    16564017
REFERENCE   2  (bases 1 to 439)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Mar 24. 124(6):1283-98."
COMMENT     Sequence Label: pLKO.1 - TRC cloning vector
FEATURES             Location/Qualifiers
     source          1..439
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    113..230
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccagagcag ccgcgtccct gcgcaaaccc agggctgcct tggaaaaggc gcaaccccaa
       61 ccccctcgag ccgcggccaa agtggatctc tgctgtccct gtaataaacc cgaaaatttt
      121 gaatttttgt aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta
      181 ctattctttc ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga
      241 atactgccat ttgtctcgag gtcgagaatt ctacaaaaaa gagcaagaag ctaaaaaaga
      301 tttaaaaatt atttttagcg cagttaatgg aacaggaact aaatttaccc caaaaatatt
      361 acgtgaatca ggatataacg ttattgaggt tgaagagcat gcatttgaag atgaaacatt
      421 taaaaatgtt gtaaatcca
//
