LOCUS       sequence-132381-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBC
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..244
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             245..472
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             473..700
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 agaaggagat atacatatgc agatctttgt gaaaacctta actggtaaga ccatcaccct
       61 ggaggtcgag cccagtgaca ccattgagaa tgtcaaggca aagatccagg acaaggaggg
      121 catcccccct gaccagcaga ggctgatctt tgcaggcaag cagctggaag atggccgcac
      181 cctgtcagac tacaacatcc agaaagagtc caccctgcac ctggtccttc gcctcagagg
      241 tggcatgcag atctttgtga agaccctgac aggcaagacc atcaccctgg aggtcgagcc
      301 cagtgacacc atagagaatg tcaaggcaaa gatccaggac aaggagggca tcccccctga
      361 tcagcagagg ctgatctttg caggcaagca gctggaagat ggccgcaccc tgtcagacta
      421 caacatccag aaagagtcca ccctgcacct ggtccttcgc ctcagaggtg gcatgcagat
      481 ctttgtgaag accctgacag gcaagaccat caccctggag gtcgagccca gtgacaccat
      541 agagaatgtc aaggcaaaga tccaggacaa ggagggcatc ccccctgacc agcagaggct
      601 gatctttgca ggcaagcagc tggaagatgg ccgcaccctg tcagactaca acatccagaa
      661 agagtccacc ctgcacctgg tccttcgcct cagaggtggg atgcagatct ttgtgaagac
      721 cctgacaggc aagaccatca ccctggaggt cgagcccagt gacaccatag agaatgtcaa
      781 ggcaa
//
