LOCUS       sequence-132383-         583 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into 
            plants by agrobacterium mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGEB31
FEATURES             Location/Qualifiers
     source          1..583
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(300..320)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(327..392)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(437..461)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(485..531)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     primer_bind     complement(520..540)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 accttggcca tctcgttgct gaagatctct tgcagatagc agatccggtt cttccgtctg
       61 gtgtatcttc ttctggcggt tctcttcagc cgggtggcct cggctgtttc gccgctgtcg
      121 aacagcaggg ctccgatcag gttcttcttg atgctgtgcc ggtcggtgtt gcccagcacc
      181 ttgaatttct tgctgggcac cttgtactcg tcggtgatca cggcccagcc cacagagttg
      241 gtgccgatgt ccaggccgat gctgtacttc ttgtcggctg ctgggactcc gtggataccg
      301 accttccgct tcttctttgg ggccatctta tcgtcatcgt ctttgtaatc aatatcatga
      361 tccttgtagt ctccgtcgtg gtccttatag tccatggtta agtatttcct tagagaattg
      421 gttctatctc cttcgaagcc tgcttttttg tacaaacttg ttgataactc tagagtcccc
      481 cgtgttctct ccaaatgaaa tgaacttcct tatatagagg aagggtcttg cgaaggatag
      541 tgggattgtg cgtcatccct tacgtcagtg gagatatcac atc
//
