LOCUS       sequence-132385-         787 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with eEF1delta core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pSTARR-seq_fly-eEF1delta
FEATURES             Location/Qualifiers
     source          1..787
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     561..589
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          634..700
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      706..715
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gggcagattg tgtggacagg taatggttgt ctggtaaaag gacagggcca tcgccaattg
       61 gagtattttg ttgataatgg tctgctagtt gaacgcttcc atcttcaatg ttgtggcggg
      121 tcttgaagtt cgctttgatt ccattctttt gtttgtctgc catgatgtat acattgtgtg
      181 agttatagtt gtattccaat ttgtgtccca gaatgttgcc atcttccttg aagtcaatac
      241 cttttaactc gattctatta acaagggtat caccttcaaa cttgacttca gcacgtgtct
      301 tgtagttgcc gtcatctttg aagaagatgg tcctttcctg tacataacct tcgggcatgg
      361 cactcttgaa aaagtcatgc cgtttcatat gatccgggta tcttgaaaag cattgaacac
      421 catagcacag agtagtgact agtgttggcc agggaacagg cagtttgcca gtagtgcaga
      481 tgaacttcag ggtaagtttt ccgtatgttg catcaccttc accctctcca ctgacagaga
      541 acttgtggcc gttaacatca ccatctaatt caacaagaat tgggacaact ccagtgaaga
      601 gttcttctcc tttgctagcc atgcttaagt ggcctgaagt aaaggataag aattagggtt
      661 agtcgtttcg gtgtgcctag gcatcagtgg ttgaacctac cttttccatg gtggctttac
      721 caacagtacc ggaatgcccc tgcaggaaca cgttgcacaa ttggctgaac aatacgataa
      781 cacgttt
//
