LOCUS       sequence-132386-         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(Y305P)
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..244
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             245..472
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             473..700
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             701..928
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 agaaggagat atacatatgc agatcttcgt gaagaccctg accggcaaga ccatcaccct
       61 agaggtggag cccagtgaca ccatcgagaa cgtgaaggcc aagatccagg ataaagaggg
      121 catcccccct gaccagcaga ggctgatctt tgccggcaag cagctggaag atggccgcac
      181 cctctctgat tacaacatcc agaaagagtc aaccctgcac ctggtcctcc gtctgagggg
      241 tggcatgcag atcttcgtga agaccctgac cggcaagacc atcaccctgg aggtggagcc
      301 cagtgacacc atcgagaatg tgaaggccaa gatccaggat aaagagggca tcccccctga
      361 ccagcagagg ctgatctttg ccggcaagca gctggaagat ggccgcaccc tctctgatta
      421 caacatccag aaggagtcaa ccctgcacct ggtcctccgt ctgaggggtg gcatgcagat
      481 cttcgtgaag accctgactg gcaagaccat caccctggag gtggagccca gtgacaccat
      541 cgagaacgtg aaggccaaga tccaggataa agagggcatc ccccctgacc agcagaggct
      601 gatctttgcc ggcaagcagc tggaagatgg ccgcaccctc tctgattaca acatccagaa
      661 ggagtcaacc ctgcacctgg tccttcgcct gagaggtggc atgcagatct tcgtgaagac
      721 cctgaccggc aagaccatca ccctggaggt ggagcccagt gacaccatcg agaatgtgaa
      781 ggccaagatc caggataaag agggcatccc ccctgaccag cagaggctga tctttgccgg
      841 caagcagctg gaagatggcc gcactctctc tgattacaac atccagaaag agtcgaccct
      901 gcacctggtc ctccgtctga ggggtggtcc t
//
