LOCUS       sequence-132389-         624 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Binary vector derived from pRGE31. Deliver sgRNA and Cas9 into 
            plants by agrobacterium mediated transformation..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Xie K, Yang Y
  TITLE     RNA-Guided Genome Editing in Plants Using a CRISPR-Cas System.
  JOURNAL   Mol Plant. 2013 Nov;6(6):1975-83. doi: 10.1093/mp/sst119. Epub 2013 
            Aug 17.
  PUBMED    23956122
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1093/mp/sst119";
            journalName: "Mol Plant"; date: "2013-11"; volume: "6"; issue: "6"; 
            pages: "1975-83"
COMMENT     Sequence Label: pRGEB31
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        325..400
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 gatcttggag gaatcagatg tgcagtcagg gaccatagca caagacaggc gtcttctact
       61 ggtgctacca gcaaatgctg gaagccggga acactgggta cgttggaaac cacgtgatgt
      121 gaagaagtaa gataaactgt aggagaaaag catttcgtag tgggccatga agcctttcag
      181 gacatgtatt gcagtatggg ccggcccatt acgcaattgg acgacaacaa agactagtat
      241 tagtaccacc tcggctatcc acatagatca aagctgattt aaaagagttg tgcagatgat
      301 ccgtggcagg agaccgaggt ctcggtttta gagctagaaa tagcaagtta aaataaggct
      361 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgttt tagagctaga
      421 aatagcaagt taaaataagg ctagtccgtt tttagcgcgt gcatgcctgc aggtccccag
      481 attagccttt tcaatttcag aaagaatgct aacccacaga tggttagaga ggcttacgca
      541 gcagcactca tcaagacgat ctacccgagc aataatctcc aggaaatcaa ataccttccc
      601 aagaaggtta aagatgcagt caaa
//
