LOCUS       sequence-132390-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with NipB core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pSTARR-seq_fly-NipB
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      193..202
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          208..274
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(319..347)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc ccgggctcga gatctgcgat
       61 caaaggagta ttatatatga agtattaacg tttcggtctg aaaaggtatt tttttattaa
      121 gtttagcgcg gccacactat cctacaggtc ggccatattc tcctgcaggg gcattccggt
      181 actgttggta aagccaccat ggaaaaggta ggttcaacca ctgatgccta ggcacaccga
      241 aacgactaac cctaattctt atcctttact tcaggccact taagcatggc tagcaaagga
      301 gaagaactct tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaacggc
      361 cacaagttct ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctg
      421 aagttcatct gcactactgg caaactgcct gttccctggc caacactagt cactactctg
      481 tgctatggtg ttcaatgctt ttcaagatac ccggatcata tgaaacggca tgactttttc
      541 aagagtgcca tgcccgaagg ttatgtacag gaaaggacca tcttcttcaa agatgacggc
      601 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      661 ttaaaaggta ttgacttcaa ggaagatggc aacattctgg gacacaaatt ggaatacaac
      721 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagcgaac
      781 ttcaagaccc gccacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      841 aatactccaa ttggcgatgg ccctgtcctt
//
