LOCUS       sequence-132391-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with NipB core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pSTARR-seq_fly-NipB
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     563..591
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          636..702
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      708..717
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
       61 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtggcg
      121 ggtcttgaag ttcgctttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      181 tgagttatag ttgtattcca atttgtgtcc cagaatgttg ccatcttcct tgaagtcaat
      241 accttttaac tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt
      301 cttgtagttg ccgtcatctt tgaagaagat ggtcctttcc tgtacataac cttcgggcat
      361 ggcactcttg aaaaagtcat gccgtttcat atgatccggg tatcttgaaa agcattgaac
      421 accatagcac agagtagtga ctagtgttgg ccagggaaca ggcagtttgc cagtagtgca
      481 gatgaacttc agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga
      541 gaacttgtgg ccgttaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      601 gagttcttct cctttgctag ccatgcttaa gtggcctgaa gtaaaggata agaattaggg
      661 ttagtcgttt cggtgtgcct aggcatcagt ggttgaacct accttttcca tggtggcttt
      721 accaacagta ccggaatgcc cctgcaggag aatatggccg acctgtagga tagtgtggcc
      781 gcgctaaact taataa
//
