LOCUS       sequence-132397-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with RpS12 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pSTARR-seq_fly-RpS12
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     562..590
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          635..701
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      707..716
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 agggcagatt gtgtggacag gtaatggttg tctggtaaaa ggacagggcc atcgccaatt
       61 ggagtatttt gttgataatg gtctgctagt tgaacgcttc catcttcaat gttgtggcgg
      121 gtcttgaagt tcgctttgat tccattcttt tgtttgtctg ccatgatgta tacattgtgt
      181 gagttatagt tgtattccaa tttgtgtccc agaatgttgc catcttcctt gaagtcaata
      241 ccttttaact cgattctatt aacaagggta tcaccttcaa acttgacttc agcacgtgtc
      301 ttgtagttgc cgtcatcttt gaagaagatg gtcctttcct gtacataacc ttcgggcatg
      361 gcactcttga aaaagtcatg ccgtttcata tgatccgggt atcttgaaaa gcattgaaca
      421 ccatagcaca gagtagtgac tagtgttggc cagggaacag gcagtttgcc agtagtgcag
      481 atgaacttca gggtaagttt tccgtatgtt gcatcacctt caccctctcc actgacagag
      541 aacttgtggc cgttaacatc accatctaat tcaacaagaa ttgggacaac tccagtgaag
      601 agttcttctc ctttgctagc catgcttaag tggcctgaag taaaggataa gaattagggt
      661 tagtcgtttc ggtgtgccta ggcatcagtg gttgaaccta ccttttccat ggtggcttta
      721 ccaacagtac cggaatgccc ctgcagggag aaataaaatc caagcatctg tacctt
//
