LOCUS       sequence-132400-         946 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 946)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(SnaBI)
FEATURES             Location/Qualifiers
     source          1..946
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..250
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             251..478
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             479..706
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             707..934
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 actttaagaa ggagatatac atatgcagat cttcgtgaag accctgaccg gcaagaccat
       61 caccctagag gtggagccca gtgacaccat cgagaacgtg aaggccaaga tccaggataa
      121 agagggcatc ccccctgacc agcagaggct gatctttgcc ggcaagcagc tggaagatgg
      181 ccgcaccctc tctgattaca acatccagaa agagtcaacc ctgcacctgg tcctccgtct
      241 gaggggtggc atgcagatct tcgtgaagac cctgaccggc aagaccatca ccctggaggt
      301 ggagcccagt gacaccatcg agaatgtgaa ggccaagatc caggataaag agggcatccc
      361 ccctgaccag cagaggctga tctttgccgg caagcagctg gaagatggcc gcaccctctc
      421 tgattacaac atccagaagg agtcaaccct gcacctggtc ctccgtctga ggggtggcat
      481 gcagatcttc gtgaagaccc tgactggcaa gaccatcacc ctggaggtgg agcccagtga
      541 caccatcgag aacgtgaagg ccaagatcca ggataaagag ggcatccccc ctgaccagca
      601 gaggctgatc tttgccggca agcagctgga agatggccgc accctctctg attacaacat
      661 ccagaaggag tcaaccctgc acctggtcct tcgcctgaga ggtggcatgc agatcttcgt
      721 gaagaccctg accggcaaga ccatcaccct ggaggtggag cccagtgaca ccatcgagaa
      781 tgtgaaggcc aagatccagg ataaagaggg catcccccct gaccagcaga ggctgatctt
      841 tgccggcaag cagctggaag atggccgcac tctctctgat tacaacatcc agaaagagtc
      901 gaccctgcac ctggtcctcc gtctgagggg tggctacgta ttattc
//
