LOCUS       sequence-132402-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with x16 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pSTARR-seq_fly-x16
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      194..203
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          209..275
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     primer_bind     complement(320..348)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc ccgggctcga gatctgcgat
       61 ccttttgctg accgttaccc gcggattgag cataactttt ggtatatttt tagcgagtcc
      121 gcagtttctt ttaatggtct gtccaacggt cactctgttt gccctgcagg ggcattccgg
      181 tactgttggt aaagccacca tggaaaaggt aggttcaacc actgatgcct aggcacaccg
      241 aaacgactaa ccctaattct tatcctttac ttcaggccac ttaagcatgg ctagcaaagg
      301 agaagaactc ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaacgg
      361 ccacaagttc tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct
      421 gaagttcatc tgcactactg gcaaactgcc tgttccctgg ccaacactag tcactactct
      481 gtgctatggt gttcaatgct tttcaagata cccggatcat atgaaacggc atgacttttt
      541 caagagtgcc atgcccgaag gttatgtaca ggaaaggacc atcttcttca aagatgacgg
      601 caactacaag acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga
      661 gttaaaaggt attgacttca aggaagatgg caacattctg ggacacaaat tggaatacaa
      721 ctataactca cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagcgaa
      781 cttcaagacc cgccacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca
      841 a
//
