LOCUS       sequence-132404-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmRPS27a
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..250
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             540..557
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(610..628)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      624..671
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(795..814)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcagat tttcgtgaaa acccttacgg ggaagaccat
       61 caccctcgag gttgaaccct cggatacgat agaaaatgta aaggccaaga tccaggataa
      121 ggaaggaatt cctcctgatc agcagagact gatctttgct ggcaagcagc tggaagatgg
      181 acgtactttg tctgactaca atattcaaaa ggagtctact cttcatcttg tgttgagact
      241 tcgtggtggt gctaagaaaa ggaagaagaa gtcttacacc actcccaaga agaataagca
      301 caagagaaag aaggttaagc tggctgtcct gaaatattat aaggtggatg agaatggcaa
      361 aattagtcgc cttcgtcgag agtgcccttc tgatgaatgt ggtgctgggg tgtttatggg
      421 aagtcacttt gacagacatt attgtggcaa atgttgtctg acttactgtt tcaacaaacc
      481 agaagacaag taaggatccg aattcgagct ccgtcgacaa gcttgcggcc gcactcgagc
      541 accaccacca ccaccactga gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg
      601 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
      661 ggggtttttt gctgaaagga ggaactatat ccggattggc gaatgggacg cgccctgtag
      721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      841 tccc
//
