LOCUS       sequence-132406-         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human MAPK1 [NM_002745.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E125 Hs.MAPK1
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(186..221)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gattaagatc tgtatcctgg ctggaatcta gcagtctctt caaaaattag ttctttgagc
      241 ttttccttag gcaagtcatc caattccatg tcgaacttga atggtgcttc ggcgatgggc
      301 tcgtcactcg ggtcgtaata ctgctccaga tatgggtggg ccagagcctg ttctacttca
      361 atcctcttgt gtgggttgaa tgtcaacatt ttgtccaata agtccagagc tttggagtca
      421 gcatttggga acagcctgtt ccatggcacc ttatttttgt gtggaagaga aagcaaatag
      481 ttcctagctt ttaaatttat tatacaattc aggtcttctt gtgatgggga tccaagaata
      541 cccaaaatgt ggttcagctg gtcaagataa tgcttccctg gaaagatggg cctgttagaa
      601 agcatttctg ccagaatgca gcctacagac caaatatcaa tggacttggt gtagcccttg
      661 gaattcaaca taatttctgg agccctgtac caacgtgtgg ccacatattc tgtcaggaac
      721 cctgtgtgat catggtctgg atctgcaaca cgggccaggc caaagtcaca gatcttgaga
      781 tcacaggtgg tgttgagcag caggttggaa ggcttgaggt cacggtgcag aacgttagct
      841 gaatggatat attttaaccc tctgaggatc tggtagagaa aatagcagat atggtcattg
      901 ctgaggtgtt gtgtcttcaa gagcttgtaa agatctgttt ccatgaggtc ctgtactata
      961 tatacatctt tcatttgc
//
