LOCUS       sequence-132407-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-Mm/HsUBA52
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..250
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             456..473
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(526..544)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      540..587
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(711..730)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 actttaagaa ggagatatac atatgcagat ctttgtgaag accctcactg gcaaaaccat
       61 cacccttgag gtcgagccca gtgacaccat tgagaatgtc aaagccaaaa ttcaagacaa
      121 ggagggtatc ccacctgacc agcagcgtct gatatttgcc ggcaaacagc tggaggatgg
      181 ccgcactctc tcagactaca acatccagaa agagtccacc ctgcacctgg tgttgcgcct
      241 gcgaggtggc attattgagc cttctctccg ccagcttgcc cagaaataca actgcgacaa
      301 gatgatctgc cgcaagtgct atgctcgcct tcaccctcgt gctgtcaact gccgcaagaa
      361 gaagtgtggt cacaccaaca acctgcgtcc caagaagaag gtcaaataag gatccgaatt
      421 cgagctccgt cgacaagctt gcggccgcac tcgagcacca ccaccaccac cactgagatc
      481 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
      541 tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa
      601 ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      661 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      721 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      781 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccc
//
