LOCUS       sequence-132408-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-DSCP-Gateway
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    302..393
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(334..353)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    407..455
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(723..744)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggcgccgggc ctttctttat gtttttggcg tcttccatgg tggctttacc aacagtaccg
       61 gaatgccgtt tggtatgcgt cttgtgattc aaagttggct tattcaaagg atattaacga
      121 aggcagcggc acgtctgctc ggctcgaaca ttcattcaca actgatgctc tcagccaccc
      181 cgcgcccttt tataccgctg cgctcgatcc ccgggcgagc tcgatcgcag atctcgagcc
      241 agacgtctga tgggaattcc catagcatgc atggacgcgt aagagctcgg tacctatcga
      301 tagagaaatg ttctggcacc tgcacttgca ctggggacag cctattttgc tagtttgttt
      361 tgtttcgttt tgttttgatg gagagcgtat gttagtacta tcgattcaca caaaaaacca
      421 acacacagat gtaatgaaaa taaagatatt ttattgcggc cgctccaagt acctcccgta
      481 ccttaatatt acttacttat catggtagct tgggctggcg taatagcgaa gaggcccgca
      541 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcaaatt gtaagcgtta
      601 atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
      661 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg
      721 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
//
