LOCUS       sequence-132410-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-DSCP-Gateway
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    122..243
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(133..152)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     187..206
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    478..602
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(627..632,633..650,651..657)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 ggtcttaccg gaaaactcga cgcaagaaaa atcagagaga tcctcataaa ggccaagaag
       61 ggcggaaaga tcgccgtgta attctagagt cggggcggcc ggccgcttcg agcagacatg
      121 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
      181 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
      241 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt
      301 ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc gtcgaccgat
      361 gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
      421 cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcaca
      481 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      541 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      601 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      661 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      721 aaatcactgg atataccacc gttgatatat
//
