LOCUS       sequence-132411-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA to the vesicular GABA transporter VGAT. Cre 
            dependent expression by flex switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pAAV-hsyn-flex-dsRed-shvgat
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..78)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(129..162)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     ncRNA           complement(329..455)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(483..497)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(525..646)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(758..781)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 agcgcagtcg agaaggtacc ggatcctcta gagtcgactc cggaataact tcgtatagga
       61 tactttatac gaagttatgc agaatggtag ctggattgta gctgctatta gcaatatgaa
      121 acctcttaat aacttcgtat agcatacatt atacgaagtt atggcgcgcc gaccaacgtc
      181 tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac caggatttat
      241 ttattctgcg aagtgatctt ccgtcacagg tatttattcg gtcgaacgcg tcgccgcgtg
      301 tttaaacgca ttagtcttcc aattgaaaaa agtgatttaa tttataccat tttaattcag
      361 ctttgtaaaa atgtatcaaa gagatagcaa ggtattcagt tttagtaaac aagataattg
      421 ctcctaaagt agccccttga attccgaggc agtaggcaaa ctcatcttgt gcaatgtatc
      481 tacatctgtg gcttcactag atacattgca caagatgagt gcgctcactg tcaacagcaa
      541 tataccttct cgagccttct gttgggttaa cctgaagaag taatcccagc aagtgtttcc
      601 aagatgtgca ggcaacgatt ctgtaaagta ctgaagcctc attcaacata gtatatgtgc
      661 tgccgaagcg agcacttaac aaggcttgcg gccgcctaca ggaacaggtg gtggcggccc
      721 tcggcgcgct cgtactgctc cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc
      781 ttggagtcca cgtagtagta gccgggcagc tgcacgggct tcttg
//
