LOCUS       sequence-132413-         804 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 804)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBC
FEATURES             Location/Qualifiers
     source          1..804
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(254..481)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(482..709)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctcgaat tcggatccac acctccccca tcacacccaa gaacaagcac
      121 aaggagggct ctttttagat actgtggtga ggaaggtacg tctgtcttcc tgtttgacct
      181 tcttggtggt aacactgggc tcgacgtcca gggtgatggt cttgcctgtc agggtcttca
      241 caaagatctg catgccacct ctgaggcgaa ggaccaggtg cagggtggac tctttctgga
      301 tgttgtagtc tgacagggtg cggccatctt ccagctgctt gcctgcaaag atcagcctct
      361 gctggtcagg ggggatgccc tccttgtcct ggatctttgc cttgacattc tctatggtgt
      421 cactgggctc gacctccagg gtgatggtct tgcctgtcag ggtcttcaca aagatctgca
      481 tgccacctct gaggcgaagg accaggtgca gggtggactc tttctggatg ttgtagtctg
      541 acagggtgcg gccatcttcc agctgcttgc ctgcaaagat cagcctctgc tggtcagggg
      601 ggatgccctc cttgtcctgg atctttgcct tgacattctc aatggtgtca ctgggctcga
      661 cctccagggt gatggttttg cctgtcaggg tcttcacaaa gatctgcatc ccacctctga
      721 ggcgaaggac taagtgcagg gtggactctt tctggatgtt gtagtctgac agggtgcggc
      781 catcttccag ctgcttgcct gcaa
//
