LOCUS       sequence-132415-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the RpS12 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-RpS12-Gateway
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    131..252
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(142..161)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     196..215
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     protein_bind    487..611
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(636..641,642..659,660..666)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 gtaccgaaag gtcttaccgg aaaactcgac gcaagaaaaa tcagagagat cctcataaag
       61 gccaagaagg gcggaaagat cgccgtgtaa ttctagagtc ggggcggccg gccgcttcga
      121 gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
      181 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
      241 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
      301 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga taaggatccg
      361 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat
      421 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc
      481 ggcagcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat
      541 atattaaatt agattttgca taaaaaacag actacataat actgtaaaac acaacatatc
      601 cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt
      661 ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa
      721 tggagaaa
//
