LOCUS       sequence-132416-         750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette downstream of 
            the firefly luciferase gene and the RpS12 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 750)
  AUTHORS   Zabidi MA, Arnold CD, Schernhuber K, Pagani M, Rath M, Frank O, 
            Stark A
  TITLE     Enhancer-core-promoter specificity separates developmental and 
            housekeeping gene regulation.
  JOURNAL   Nature. 2015 Feb 26;518(7540):556-9. doi: 10.1038/nature13994. Epub 
            2014 Dec 15.
  PUBMED    25517091
REFERENCE   2  (bases 1 to 750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature13994"; journalName: "Nature"; date: "2015-02-26- 
            26"; volume: "518"; issue: "7540"; pages: "556-9"
COMMENT     Sequence Label: pGL3-RpS12-Gateway
FEATURES             Location/Qualifiers
     source          1..750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    303..394
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(335..354)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     polyA_signal    408..456
                     /note="synthetic polyadenylation signal"
     primer_bind     complement(724..745)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ccgggccttt ctttatgttt ttggcgtctt ccattgttct gcggttaagg atgcactgca
       61 ctggaaaaga tagttaattg ttcatattta ttacattttc gataagaaaa cctcttcatt
      121 tcggagaaat aaaatccaag catctgtacc ttttgacaac cggaaagaaa gagaacaaaa
      181 tcggcatggc gctggagatg tgagttttta gctattggta caagattgca gatctcgagc
      241 cagacgtctg atgggaattc ccatagcatg catggacgcg taagagctcg gtacctatcg
      301 atagagaaat gttctggcac ctgcacttgc actggggaca gcctattttg ctagtttgtt
      361 ttgtttcgtt ttgttttgat ggagagcgta tgttagtact atcgattcac acaaaaaacc
      421 aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccgctccaag tacctcccgt
      481 accttaatat tacttactta tcatggtagc ttgggctggc gtaatagcga agaggcccgc
      541 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcaaat tgtaagcgtt
      601 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
      661 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
      721 gttccagttt ggaacaagag tccactatta
//
