LOCUS       sequence-132417-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..33)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(99..326)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(327..554)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(555..782)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 agcagccgga tctcagtggt ggtggtggtg gtgctcgagt gcggccgcaa gcttgtcgac
       61 ggagctcgaa ttcggatccg cagaccgaat aattaatagc cacccctcag acggaggacc
      121 aggtgcaggg tcgactcttt ctggatgttg taatcagaga gagtgcggcc atcttccagc
      181 tgcttgccgg caaagatcag cctctgctgg tcagggggga tgccctcttt atcctggatc
      241 ttggccttca cattctcgat ggtgtcactg ggctccacct ccagggtgat ggtcttgccg
      301 gtcagggtct tcacgaagat ctgcatgcca cctctcaggc gaaggaccag gtgcagggtt
      361 gactccttct ggatgttgta atcagagagg gtgcggccat cttccagctg cttgccggca
      421 aagatcagcc tctgctggtc aggggggatg ccctctttat cctggatctt ggccttcacg
      481 ttctcgatgg tgtcactggg ctccacctcc agggtgatgg tcttgccagt cagggtcttc
      541 acgaagatct gcatgccacc cctcagacgg aggaccaggt gcagggttga ctccttctgg
      601 atgttgtaat cagagagggt gcggccatct tccagctgct tgccggcaaa gatcagcctc
      661 tgctggtcag gggggatgcc ctctttatcc tggatcttgg ccttcacatt ctcgatggtg
      721 tcactgggct ccacctccag ggtgatggtc ttgccggtca gggtcttcac gaagatctgc
      781 atgccacccc tcagacggag gaccaggtgc agggttgact ctttctggat gttgta
//
