LOCUS       sequence-132419-         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(Y305P)
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(19..36)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(64..291)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(292..519)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(520..747)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 gttagcagcc ggatctcagt ggtggtggtg gtggtgctcg agtgcggccg cggtacctta
       61 aggaccaccc ctcagacgga ggaccaggtg cagggtcgac tctttctgga tgttgtaatc
      121 agagagagtg cggccatctt ccagctgctt gccggcaaag atcagcctct gctggtcagg
      181 ggggatgccc tctttatcct ggatcttggc cttcacattc tcgatggtgt cactgggctc
      241 cacctccagg gtgatggtct tgccggtcag ggtcttcacg aagatctgca tgccacctct
      301 caggcgaagg accaggtgca gggttgactc cttctggatg ttgtaatcag agagggtgcg
      361 gccatcttcc agctgcttgc cggcaaagat cagcctctgc tggtcagggg ggatgccctc
      421 tttatcctgg atcttggcct tcacgttctc gatggtgtca ctgggctcca cctccagggt
      481 gatggtcttg ccagtcaggg tcttcacgaa gatctgcatg ccacccctca gacggaggac
      541 caggtgcagg gttgactcct tctggatgtt gtaatcagag agggtgcggc catcttccag
      601 ctgcttgccg gcaaagatca gcctctgctg gtcagggggg atgccctctt tatcctggat
      661 cttggccttc acattctcga tggtgtcact gggctccacc tccagggtga tggtcttgcc
      721 ggtcagggtc ttcacgaaga tctgcatgcc acccctcaga cggaggacca ggtgcagggt
      781 tgactctttc tggatgttgt aatcagagag ggtgcg
//
