LOCUS       sequence-132421-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq screening vector with hsp70 core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pSTARR-seq_fly-hsp70
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     563..591
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     intron          639..705
                     /label=Mhc intron
                     /note="modified Drosophila myosin heavy chain intron 16 
                     (Pfeiffer et al., 2010)"
     regulatory      711..720
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aagggcagat tgtgtggaca ggtaatggtt gtctggtaaa aggacagggc catcgccaat
       61 tggagtattt tgttgataat ggtctgctag ttgaacgctt ccatcttcaa tgttgtggcg
      121 ggtcttgaag ttcgctttga ttccattctt ttgtttgtct gccatgatgt atacattgtg
      181 tgagttatag ttgtattcca atttgtgtcc cagaatgttg ccatcttcct tgaagtcaat
      241 accttttaac tcgattctat taacaagggt atcaccttca aacttgactt cagcacgtgt
      301 cttgtagttg ccgtcatctt tgaagaagat ggtcctttcc tgtacataac cttcgggcat
      361 ggcactcttg aaaaagtcat gccgtttcat atgatccggg tatcttgaaa agcattgaac
      421 accatagcac agagtagtga ctagtgttgg ccagggaaca ggcagtttgc cagtagtgca
      481 gatgaacttc agggtaagtt ttccgtatgt tgcatcacct tcaccctctc cactgacaga
      541 gaacttgtgg ccgttaacat caccatctaa ttcaacaaga attgggacaa ctccagtgaa
      601 gagttcttct cctttgctag ccatgcttaa gctttggcct gaagtaaagg ataagaatta
      661 gggttagtcg tttcggtgtg cctaggcatc agtggttgaa cctacctttt ccatggtggc
      721 tttaccaaca gtaccggaat gccaagcttc tgcagattgt ttagcttgtt cagctgcgct
      781 tgtttatttg cttagcttt
//
