LOCUS       sequence-132422-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmUBB(SnaBI)
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..40)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(106..333)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             complement(334..561)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
ORIGIN
        1 ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg gccgcaagct
       61 tgtcgacgga gctcgaattc ggatccgcag accgaataat acgtagccac ccctcagacg
      121 gaggaccagg tgcagggtcg actctttctg gatgttgtaa tcagagagag tgcggccatc
      181 ttccagctgc ttgccggcaa agatcagcct ctgctggtca ggggggatgc cctctttatc
      241 ctggatcttg gccttcacat tctcgatggt gtcactgggc tccacctcca gggtgatggt
      301 cttgccggtc agggtcttca cgaagatctg catgccacct ctcaggcgaa ggaccaggtg
      361 cagggttgac tccttctgga tgttgtaatc agagagggtg cggccatctt ccagctgctt
      421 gccggcaaag atcagcctct gctggtcagg ggggatgccc tctttatcct ggatcttggc
      481 cttcacgttc tcgatggtgt cactgggctc cacctccagg gtgatggtct tgccagtcag
      541 ggtcttcacg aagatctgca tgccacccct cagacggagg accaggtgca gggttgactc
      601 cttctggatg ttgtaatcag agagggtgcg gccatcttcc agctgcttgc cggcaaagat
      661 cagcctctgc tggtcagggg ggatgccctc tttatcctgg atcttggcct tcacattctc
      721 gatggtgtca ctgggctcca cctccagggt gatggtcttg ccggtcagg
//
