LOCUS       sequence-132425-         597 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses scramble shRNA Cre-dependently by flex switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 597)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pAAV-hysn-flex-dsRed-shscramble
FEATURES             Location/Qualifiers
     source          1..597
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(45..78)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(129..162)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     ncRNA           complement(328..454)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(481..495)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 agcgcagtcg agaaggtacc ggatcctcta gagtcgactc cggaataact tcgtatagga
       61 tactttatac gaagttatgc agaatggtag ctggattgta gctgctatta gcaatatgaa
      121 acctcttaat aacttcgtat agcatacatt atacgaagtt atggcgcgcc atcaacgtct
      181 cattttcgcc aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt
      241 tattctgcga agtgatcttc cgtcacaggt atttattcgg tcgaacgcgt cgccgcgtgt
      301 ttaaacgcat tagtcttcca attgaaaaaa gtgatttaat ttataccatt ttaattcagc
      361 tttgtaaaaa tgtatcaaag agatagcaag gtattcagtt ttagtaaaca agataattgc
      421 tcctaaagta gccccttgaa ttccgaggca gtaggcagcc gcgattaggc tgttataata
      481 catctgtggc ttcactatta taacagccta atcgcggccg ctcactgtca acagcaatat
      541 accttctcga gccttctgtt gggttaacct gaagaagtaa tcccagcaag tgtttcc
//
