LOCUS       sequence-132427-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  flySTARR-seq validation vector with Gateway cassette upstream of the
            firefly luciferase gene and the DSCP core promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Arnold CD, Gerlach D, Stelzer C, Boryn LM, Rath M, Stark A
  TITLE     Genome-wide quantitative enhancer activity maps identified by 
            STARR-seq.
  JOURNAL   Science. 2013 Mar 1;339(6123):1074-7. doi: 10.1126/science.1232542. 
            Epub 2013 Jan 17.
  PUBMED    23328393
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.1232542"; journalName: "Science"; date: "2013-03-1-
            1"; volume: "339"; issue: "6123"; pages: "1074-7"
COMMENT     Sequence Label: pGL3-Gateway-DSCP
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    234..358
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(399..704)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(501..520)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gcgccgggcc tttctttatg tttttggcgt cttccatggt ggctttacca acagtaccgg
       61 aatgccgttt ggtatgcgtc ttgtgattca aagttggctt attcaaagga tattaacgaa
      121 ggcagcggca cgtctgctcg gctcgaacat tcattcacaa ctgatgctct cagccacccc
      181 gcgccctttt ataccgctgc gctcgatccc cgggcgagct cgatcgcaga tctaccactt
      241 tgtacaagaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
      301 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggt
      361 cgacctgcag actggctgtg tataagggag cctgacattt atattcccca gaacatcagg
      421 ttaatggcgt ttttgatgtc attttcgcgg tggctgagat cagccacttc ttccccgata
      481 acggagaccg gcacactggc catatcggtg gtcatcatgc gccagctttc atccccgata
      541 tgcaccaccg ggtaaagttc acgggagact ttatctgaca gcagacgtgc actggccagg
      601 gggatcacca tccgtcgccc gggcgtgtca ataatatcac tctgtacatc cacaaacaga
      661 cgataacggc tctctctttt ataggtgtaa accttaaact gcatttcacc agcccctgtt
      721 ctcgtcagca aaagagccgt tcatttcaat aaaccgggcg acctcagcca tcccttcctg
      781 attttccgct ttccagcgtt cggcacgcag acgacgg
//
