LOCUS       sequence-132431-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRed and scramble shRNA under the control of the CMV 
            promoter in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pPRIME-dsRed-shscramble
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..162
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           274..395
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           422..436
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           463..589
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc
       61 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
      121 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      181 agcagtacga gcgcgccgag ggccgccacc acctgttcct gtaggcggcc gcaagccttg
      241 ttaagtgctc gcttcggcag cacatatact atgttgaatg aggcttcagt actttacaga
      301 atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca
      361 gaaggctcga gaaggtatat tgctgttgac agtgagcggc cgcgattagg ctgttataat
      421 agtgaagcca cagatgtatt ataacagcct aatcgcggct gcctactgcc tcggaattca
      481 aggggctact ttaggagcaa ttatcttgtt tactaaaact gaataccttg ctatctcttt
      541 gatacatttt tacaaagctg aattaaaatg gtataaatta aatcactttt ttcaattgga
      601 agactaatgc gtttaaacac gcggcgacgc gttcgaccga ataaatacct gtgacggaag
      661 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
      721 acttttggcg aa
//
