LOCUS       sequence-132434-         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRed and shRNA against vgat under the control of the CMV 
            promoter in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pPRIME-dsRed-shvgat
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     137..160
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           272..393
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           421..435
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           463..589
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cgcgacggcg tgctgaaggg cgagatccac aaggccctga agctgaagga cggcggccac
       61 tacctggtgg agttcaagtc catctacatg gccaagaagc ccgtgcagct gcccggctac
      121 tactacgtgg actccaagct ggacatcacc tcccacaacg aggactacac catcgtggag
      181 cagtacgagc gcgccgaggg ccgccaccac ctgttcctgt aggcggccgc aagccttgtt
      241 aagtgctcgc ttcggcagca catatactat gttgaatgag gcttcagtac tttacagaat
      301 cgttgcctgc acatcttgga aacacttgct gggattactt cttcaggtta acccaacaga
      361 aggctcgaga aggtatattg ctgttgacag tgagcgcact catcttgtgc aatgtatcta
      421 gtgaagccac agatgtagat acattgcaca agatgagttt gcctactgcc tcggaattca
      481 aggggctact ttaggagcaa ttatcttgtt tactaaaact gaataccttg ctatctcttt
      541 gatacatttt tacaaagctg aattaaaatg gtataaatta aatcactttt ttcaattgga
      601 agactaatgc gtttaaacac gcggcgacgc gttcgaccga ataaatacct gtgacggaag
      661 atcacttcgc agaataaata aatcctggtg tccctgttga taccgggaag ccctgggcca
      721 acttttggcg aaaatgagac gttgatcggc acgtaagagg ttccaacttt caccataatg
      781 aaataagatc actaccgggc gtattt
//
