LOCUS       sequence-132435-         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human KRAS [NM_004985.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E115 Hs.KRAS
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(183..749)
                     /codon_start=1
                     /product="human small GTPase proto-oncoprotein"
                     /label=K-Ras
                     /translation="MTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHHYREQIKRVK
                     DSEDVPMVLVGNKCDLPSRTVDTKQAQDLARSYGIPFIETSAKTRQGVDDAFYTLVREI
                     RKHKEKMSKDGKKKKKKSKTKCVIM"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gattacataa ttacacactt tgtctttgac ttctttttct tctttttacc atctttgctc
      241 atcttttctt tatgttttcg aatttctcga actaatgtat agaaggcatc atcaacaccc
      301 tgtcttgtct ttgctgatgt ttcaataaaa ggaattccat aacttcttgc taagtcctga
      361 gcctgttttg tgtctactgt tctagaaggc aaatcacatt tatttcctac taggaccata
      421 ggtacatctt cagagtcctt aactctttta atttgttctc tataatggtg aatatcttca
      481 aatgatttag tattatttat ggcaaataca caaagaaagc cctccccagt cctcatgtac
      541 tggtccctca ttgcactgta ctcctcttga cctgctgtgt cgagaatatc caagagacag
      601 gtttctccat caattactac ttgcttcctg taggaatcct ctattgttgg atcatattcg
      661 tccacaaaat gattctgaat tagctgtatc gtcaaggcac tcttgcctac gccaccagct
      721 ccaactacca caagtttata ttcagtcatg gtgccaacc
//
