LOCUS       sequence-132443-         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmRPS27a
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..41)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(331..558)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             566..588
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(619..638)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(620..638)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagtgc ggccgcaagc
       61 ttgtcgacgg agctcgaatt cggatcctta cttgtcttct ggtttgttga aacagtaagt
      121 cagacaacat ttgccacaat aatgtctgtc aaagtgactt cccataaaca ccccagcacc
      181 acattcatca gaagggcact ctcgacgaag gcgactaatt ttgccattct catccacctt
      241 ataatatttc aggacagcca gcttaacctt ctttctcttg tgcttattct tcttgggagt
      301 ggtgtaagac ttcttcttcc ttttcttagc accaccacga agtctcaaca caagatgaag
      361 agtagactcc ttttgaatat tgtagtcaga caaagtacgt ccatcttcca gctgcttgcc
      421 agcaaagatc agtctctgct gatcaggagg aattccttcc ttatcctgga tcttggcctt
      481 tacattttct atcgtatccg agggttcaac ctcgagggtg atggtcttcc ccgtaagggt
      541 tttcacgaaa atctgcatat gtatatctcc ttcttaaagt taaacaaaat tatttctaga
      601 gggaaaccgt tgtggtctcc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg
      661 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg
      721 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg
      781 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt
      841 ttccgtgttt cgtaaagtct ggaaacgcgg a
//
