LOCUS       sequence-132444-        1026 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 1026)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-MmRPS27a
FEATURES             Location/Qualifiers
     source          1..1026
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             9..31
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             39..266
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             556..573
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(626..644)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      640..687
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(811..830)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 agaataattt tgtttaactt taagaaggag atatacatat gcagattttc gtgaaaaccc
       61 ttacggggaa gaccatcacc ctcgaggttg aaccctcgga tacgatagaa aatgtaaagg
      121 ccaagatcca ggataaggaa ggaattcctc ctgatcagca gagactgatc tttgctggca
      181 agcagctgga agatggacgt actttgtctg actacaatat tcaaaaggag tctactcttc
      241 atcttgtgtt gagacttcgt ggtggtgcta agaaaaggaa gaagaagtct tacaccactc
      301 ccaagaagaa taagcacaag agaaagaagg ttaagctggc tgtcctgaaa tattataagg
      361 tggatgagaa tggcaaaatt agtcgccttc gtcgagagtg cccttctgat gaatgtggtg
      421 ctggggtgtt tatgggaagt cactttgaca gacattattg tggcaaatgt tgtctgactt
      481 actgtttcaa caaaccagaa gacaagtaag gatccgaatt cgagctccgt cgacaagctt
      541 gcggccgcac tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga
      601 aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      661 tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat
      721 gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
      781 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      841 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      901 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      961 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     1021 gtggac
//
