LOCUS       sequence-132445-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  In vitro transcription vector to generate phiC31 mRNA (i.e. for 
            zebrafish injection)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Bischof J, Maeda RK, Hediger M, Karch F, Basler K
  TITLE     An optimized transgenesis system for Drosophila using 
            germ-line-specific phiC31 integrases.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 Feb 27;104(9):3312-7. Epub 2007 Feb 
            22.
  PUBMED    17360644
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-02-27"; volume: "104(9)"; pages: 
            "3312-7. Epub 2007 Feb 22"
COMMENT     Sequence Label: pcDNA3.1 phiC31
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             92..114
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagcttggt accgagctcg ctagaaataa ttttgtttaa ctttaagaag gagatataca
      121 tatggacacg tacgcgggtg cttacgaccg tcagtcgcgc gagcgcgaaa attcgagcgc
      181 agcaagccca gcgacacagc gtagcgccaa cgaagacaag gcggccgacc ttcagcgcga
      241 agtcgagcgc gacgggggcc ggttcaggtt cgtcgggcat ttcagcgaag cgccgggcac
      301 gtcggcgttc gggacggcgg agcgcccgga gttcgaacgc atcctgaacg aatgccgcgc
      361 cgggcggctc aacatgatca ttgtctatga cgtgtcgcgc ttctcgcgcc tgaaggtcat
      421 ggacgcgatt ccgattgtct cggaattgct cgccctgggc gtgacgattg tttccactca
      481 ggaaggcgtc ttccggcagg gaaacgtcat ggacctgatt cacctgatta tgcggctcga
      541 cgcgtcgcac aaagaatctt cgctgaagtc ggcgaagatt ctcgacacga agaaccttca
      601 gcgcgaattg ggcgggtacg tcggcgggaa ggcgccttac ggcttcgagc ttgtttcgga
      661 gacgaaggag atcacgcgca acggccgaat ggtcaatgtc gtcatcaaca agcttgcgca
      721 ctcgaccact ccccttaccg gacccttcga gttcgagccc gacgtaatcc ggtggtggtg
      781 gcgtgagatc aagacgcaca aacaccttcc cttcaagccg ggcagtcaag ccgccattca
      841 cc
//
