LOCUS       sequence-132447-        1017 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 1017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-Mm/HsUBA52
FEATURES             Location/Qualifiers
     source          1..1017
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             1..23
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             31..258
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             464..481
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(534..552)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      548..595
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      632..1011
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(719..738)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     929..950
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttgtttaac tttaagaagg agatatacat atgcagatct ttgtgaagac cctcactggc
       61 aaaaccatca cccttgaggt cgagcccagt gacaccattg agaatgtcaa agccaaaatt
      121 caagacaagg agggtatccc acctgaccag cagcgtctga tatttgccgg caaacagctg
      181 gaggatggcc gcactctctc agactacaac atccagaaag agtccaccct gcacctggtg
      241 ttgcgcctgc gaggtggcat tattgagcct tctctccgcc agcttgccca gaaatacaac
      301 tgcgacaaga tgatctgccg caagtgctat gctcgccttc accctcgtgc tgtcaactgc
      361 cgcaagaaga agtgtggtca caccaacaac ctgcgtccca agaagaaggt caaataagga
      421 tccgaattcg agctccgtcg acaagcttgc ggccgcactc gagcaccacc accaccacca
      481 ctgagatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
      541 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
      601 aggaggaact atatccggat tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
      661 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      721 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      781 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      841 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      901 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacacactca
      961 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctatg
//
