LOCUS       sequence-132453-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-HsRPS27a
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(25..42)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(332..559)
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     RBS             567..589
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(620..639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(621..639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggctttgtta gcagccggat ctcagtggtg gtggtggtgg tgctcgagtg cggccgcaag
       61 cttgtcgacg gagctcgaat tcggatcctt acttgtcttc tggtttgttg aaacagtaag
      121 tcagacaaca tttgccacaa taatgtctgt caaagtgact tgccataaac accccagcac
      181 cacattcatc agaagggcac tctcgacgaa ggcgactaat tttgccattc tcatccacct
      241 tataatattt caggacagcc agcttaacct tctttctctt gtgcttattc ttcttgggag
      301 tggtgtaaga cttcttcttc cttttcttag caccaccacg aagtctcaac acaagatgaa
      361 gagtagactc cttttgaata ttgtagtcag acaaagtacg tccatcttcc agctgcttgc
      421 cagcaaagat cagtctctgc tgatcaggag gaattccttc cttatcctgg atcttggcct
      481 ttacattttc tatcgtatcc gagggttcaa cctcgagggt gatggtcttc cccgtaaggg
      541 ttttcacgaa aatctgcata tgtatatctc cttcttaaag ttaaacaaaa ttatttctag
      601 agggaaaccg ttgtggtctc cctatagtga gtcgtattaa tttcgcggga tcgagatctc
      661 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
      721 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
      781 gtgaagcgac tgctgctgca a
//
