LOCUS       sequence-132454-         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-HsRPS27a
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             28..255
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             545..562
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(615..633)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      629..676
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(800..819)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gtttaacttt aagaaggaga tatacatatg cagattttcg tgaaaaccct tacggggaag
       61 accatcaccc tcgaggttga accctcggat acgatagaaa atgtaaaggc caagatccag
      121 gataaggaag gaattcctcc tgatcagcag agactgatct ttgctggcaa gcagctggaa
      181 gatggacgta ctttgtctga ctacaatatt caaaaggagt ctactcttca tcttgtgttg
      241 agacttcgtg gtggtgctaa gaaaaggaag aagaagtctt acaccactcc caagaagaat
      301 aagcacaaga gaaagaaggt taagctggct gtcctgaaat attataaggt ggatgagaat
      361 ggcaaaatta gtcgccttcg tcgagagtgc ccttctgatg aatgtggtgc tggggtgttt
      421 atggcaagtc actttgacag acattattgt ggcaaatgtt gtctgactta ctgtttcaac
      481 aaaccagaag acaagtaagg atccgaattc gagctccgtc gacaagcttg cggccgcact
      541 cgagcaccac caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
      601 gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
      661 cttgaggggt tttttgctga aaggaggaac tatatccgga ttggcgaatg ggacgcgccc
      721 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
      781 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
      841 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
      901 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg c
//
