LOCUS       sequence-132455-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human IRS1 [NM_005544.2] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E109 Hs.IRS1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      818..827
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gattactgac ggtcctctgg ctgcttctgg aaactgatgc tggcataggc gcttaaatcc
      241 tcacttgagc ggcgggtgga gctgctctca ccgctgccca ggggttgatg agggggtggg
      301 ggtgggggag gctgcggttc aggggtgcac tcctgagggc actgtttgaa gtccttgacc
      361 aaatccaggt ctatgtagtt aagaccattc tccaaacccc cagcagcccc acacagtttg
      421 gctggctcct tgggggctcc cccaagctcc ccaggcctca gccacacatt ctcaaaggaa
      481 gcagagctgt ggcgtttcac atcctcgctg ctgctgctgc taccgccacc gccccctact
      541 gctgcccccg ctccaaaggg cactgtgttg cccacccggg tggcactggg tgttgaggag
      601 aaagtctcgg agctatgcct ccgccggcac ccttgtgggt ctgcacggat cactttggca
      661 ctctggttgc ggttaggact gaggttcacc cgggtgaagg cgctcatgcc cccaggtcct
      721 tgtgggcccc ccagcaggga cgagtgggca gccagctctg ctgccccttg aggcccagtc
      781 ggggaagcag aggctgctga ggatgaggag gcagcagcca tggtggccct gggcaggctc
      841 acctcctctg cagcaatgcc tgttcgcatg tcagcatagc ttacaggggc agctggcgag
      901 gtgtccacgt agctctgacg gggacaactc atctgcatgg tcatgtagtc accccggctg
      961 ctgggcactg cccgggtagg cctgcaaatg ctagcag
//
