LOCUS       sequence-132456-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human IRS1 [NM_005544.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E110 Hs.IRS1-nostop
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    85..184
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      815..824
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggtgtgcgtc acccggcaac cttgggtagc agcgaagtcg aggcatttct gtcctggctg
       61 gtctagagga tctaagcttg catgcaaata atgattttat tttgactgat agtgacctgt
      121 tcgttgcaac aaattgataa gcaatgcttt cctataatgc caactttgta caagaaagtt
      181 gactgacggt cctctggctg cttctggaaa ctgatgctgg cataggcgct taaatcctca
      241 cttgagcggc gggtggagct gctctcaccg ctgcccaggg gttgatgagg gggtgggggt
      301 gggggaggct gcggttcagg ggtgcactcc tgagggcact gtttgaagtc cttgaccaaa
      361 tccaggtcta tgtagttaag accattctcc aaacccccag cagccccaca cagtttggct
      421 ggctccttgg gggctccccc aagctcccca ggcctcagcc acacattctc aaaggaagca
      481 gagctgtggc gtttcacatc ctcgctgctg ctgctgctac cgccaccgcc ccctactgct
      541 gcccccgctc caaagggcac tgtgttgccc acccgggtgg cactgggtgt tgaggagaaa
      601 gtctcggagc tatgcctccg ccggcaccct tgtgggtctg cacggatcac tttggcactc
      661 tggttgcggt taggactgag gttcacccgg gtgaaggcgc tcatgccccc aggtccttgt
      721 gggcccccca gcagggacga gtgggcagcc agctctgctg ccccttgagg cccagtcggg
      781 gaagcagagg ctgctgagga tgaggaggca gcagccatgg tggccctggg caggctcacc
      841 tcctctgcag caatgcctgt tcgcatgtca gcatagctta caggggcagc tggcgaggtg
      901 tccacgtagc tctgacgggg acaactcatc tgcatggtca tgtagtcacc ccggctgctg
      961 ggcactgccc gggtaggcct gcaaatgcta gcagccccgg gaggtgcagg gcccagtctg
     1021 cccatctcga ccccagtgct ctcctgccag g
//
