LOCUS       sequence-132458-         728 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of hUCH37 (M148D/F149D) in E.coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 728)
  AUTHORS   VanderLinden RT, Hemmis CW, Schmitt B, Ndoja A, Whitby FG, Robinson 
            H, Cohen RE, Yao T, Hill CP
  TITLE     Structural Basis for the Activation and Inhibition of the UCH37 
            Deubiquitylase.
  JOURNAL   Mol Cell. 2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016.
  PUBMED    25702872
REFERENCE   2  (bases 1 to 728)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2015 Feb 17. pii: S1097-2765(15)00017-9. doi: 
            10.1016/j.molcel.2015.01.016."
COMMENT     Sequence Label: pET151-hUCH37 (ISF1)(M148D/F149D)
FEATURES             Location/Qualifiers
     source          1..728
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..36
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..78
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 gaaggagata tacatatgca tcatcaccat caccatggta agcctatccc taaccctctc
       61 ctcggtctcg attctacgga aaacctgtat tttcagggaa ttgatccctt caccatgacg
      121 ggcaatgccg gggagtggtg cctcatggaa agcgaccccg gggtcttcac cgagctcatt
      181 aaaggattcg gttgccgagg agcccaagta gaagaaatat ggagtttaga gcctgagaat
      241 tttgaaaaat taaagccagt tcatgggtta atttttcttt tcaagtggca gccaggagaa
      301 gaaccagcag gctctgtggt tcaggactcc cgacttgaca cgatattttt tgctaagcag
      361 gtaattaata atgcttgtgc tactcaagcc atagtgagtg tgttactgaa ctgtacccac
      421 caggatgtcc atttaggcga gacattatca gagtttaaag aattttcaca aagttttgat
      481 gcagctatga aaggcttggc actgagcaat tcagatgtga ttcgacaagt acacaacagt
      541 ttcgccagac agcaagatga tgaatttgat acgaagacat cagcaaaaga agaagatgct
      601 tttcactttg tcagttatgt tcctgttaat gggagactgt atgaattaga tggattaaga
      661 gaaggaccga ttgatttagg tgcatgcaat caagatgatt ggatcagtgc agtaaggcct
      721 gtcataga
//
