LOCUS       sequence-132475-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. 
            Confers zolpidem sensitivity on zolpidem-insensitive neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Uygun DS, Ye Z, Zecharia AY, Harding EC, Yu X, Yustos R, Vyssotski 
            AL, Brickley SG, Franks NP, Wisden W
  TITLE     Bottom-Up versus Top-Down Induction of Sleep by Zolpidem Acting on 
            Histaminergic and Neocortex Neurons.
  JOURNAL   J Neurosci. 2016 Nov 2;36(44):11171-11184.
  PUBMED    27807161
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2016-11-2"; volume: "36(44)"; pages: 
            "11171-11184"
COMMENT     Sequence Label: pAAV-flex-rev-g2-2A-Venus
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    56..89
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    140..173
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gcgcgataac
       61 ttcgtataat gtatgctata cgaagttatt aagaggtttc atattgctaa tagcagctac
      121 aatccagcta ccattctgca taacttcgta taaagtatcc tatacgaagt tattccggag
      181 tcgaccttgg attgctcctc ctggcagaca gacagaggag gctggaaggc agctgtcaac
      241 agggcttctt ggtcgccttt gtctgtgctg tgcactctct ggcagccagt gcatctctta
      301 tttaaggagt tccctattcc cctcctttag ccccgccccc ataaagtccc ttccttcagg
      361 cttaatttca gcctctcttg tccccgtcct ttctgatttg aactttgatc aacagaatca
      421 gagaacatgg tgtttttaga ccagttgggg cacctagcct cccaagccta caagcagctc
      481 tggatagaca aggtaaaaga gccctctatc atccctgccc ctgcattcag accatagagc
      541 cccagccttg aataaagctc ctagctcaga ggccagaaat aggccaaggg cattcacaat
      601 aagtagcaca gagggtggag ttgctcctgc cctggcttct ctatgaggtg agggcagagc
      661 cggctcagag aagcccatag ctagccaggc tgagctcagg acagatcaac aacaggatat
      721 gaaggattct ttttatccat tactacatag cacttgttct gggttctttg cctcgactac
      781 tgtttaagct aacggaggaa a
//
