LOCUS       sequence-132476-         764 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is pAAV-panpromoter-flex-rev-gamma2F77-2A-Venus. 
            Confers zolpidem sensitivity on zolpidem-insensitive neurons..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 764)
  AUTHORS   Uygun DS, Ye Z, Zecharia AY, Harding EC, Yu X, Yustos R, Vyssotski 
            AL, Brickley SG, Franks NP, Wisden W
  TITLE     Bottom-Up versus Top-Down Induction of Sleep by Zolpidem Acting on 
            Histaminergic and Neocortex Neurons.
  JOURNAL   J Neurosci. 2016 Nov 2;36(44):11171-11184.
  PUBMED    27807161
REFERENCE   2  (bases 1 to 764)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2016-11-2"; volume: "36(44)"; pages: 
            "11171-11184"
COMMENT     Sequence Label: pAAV-flex-rev-g2-2A-Venus
FEATURES             Location/Qualifiers
     source          1..764
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(39..72)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(123..156)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
ORIGIN
        1 tccagaggtt gattatcgat aagcttgata tcgaattcat aacttcgtat agcatacatt
       61 atacgaagtt attctttgca ccattctaaa gaataacagt gataatttct gggttaaggc
      121 aaataacttc gtataggata ctttatacga agttattccg cgcggccgcg tcgacggtat
      181 cgatgcgggg aggcggccca aagggagaat atatggatcc atgacgctgt ggattctgct
      241 cctgctatcg ctctacccag gcttcacaag ccaaaagtca gatgacacct acaggtacat
      301 cgatgactat gaagattaca cttctaataa aacatgggtg ttgactccaa aagttccaga
      361 gggtgatgtc actgtcatct taaacaacct gctggaaggc tatgacaaca aacttcgacc
      421 tgacatcgga gtgaaaccaa cattaattca tacagatatg tatgtgaaca gcattggtcc
      481 agtgaatgct atcaatatgg aatatacaat tgatattttt tttgcccaaa cctggtatga
      541 cagacgtttg aaatttaaca gtaccattaa agttctccgg ttgaatagca atatggtggg
      601 gaaaatctgg attccagaca ctttcttcag gaactccaaa aaggctgatg ctcactggat
      661 caccactccc aacaggatgc tgagaatttg gaatgatggt cgagttctct acaccttaag
      721 gctaacaatt gatgctgagt gccagttgca attacacaac ttcc
//
