LOCUS       sequence-132479-         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses shRNA to the vesicular GABA transporter VGAT. Cre 
            dependent expression by flex switch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pAAV-hsyn-flex-dsRed-shvgat
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..162
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     ncRNA           274..395
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           423..437
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           465..591
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct gaagctgaag gacggcggcc
       61 actacctggt ggagttcaag tccatctaca tggccaagaa gcccgtgcag ctgcccggct
      121 actactacgt ggactccaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      181 agcagtacga gcgcgccgag ggccgccacc acctgttcct gtaggcggcc gcaagccttg
      241 ttaagtgctc gcttcggcag cacatatact atgttgaatg aggcttcagt actttacaga
      301 atcgttgcct gcacatcttg gaaacacttg ctgggattac ttcttcaggt taacccaaca
      361 gaaggctcga gaaggtatat tgctgttgac agtgagcgca ctcatcttgt gcaatgtatc
      421 tagtgaagcc acagatgtag atacattgca caagatgagt ttgcctactg cctcggaatt
      481 caaggggcta ctttaggagc aattatcttg tttactaaaa ctgaatacct tgctatctct
      541 ttgatacatt tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg
      601 gaagactaat gcgtttaaac acgcggcgac gcgttcgacc gaataaatac ctgtgacgga
      661 agatcacttc gcagaataaa taaatcctgg tgtccctgtt gataccggga agccctgggc
      721 caacttttgg cg
//
