LOCUS       sequence-132481-         670 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRed and scramble shRNA under the control of the CMV 
            promoter in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pPRIME-dsRed-shscramble
FEATURES             Location/Qualifiers
     source          1..670
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(20..122)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     ncRNA           complement(276..402)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(429..443)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(470..591)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
ORIGIN
        1 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgac aactcaaaaa
       61 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgccgat
      121 caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca gggacaccag
      181 gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggtc gaacgcgtcg
      241 ccgcgtgttt aaacgcatta gtcttccaat tgaaaaaagt gatttaattt ataccatttt
      301 aattcagctt tgtaaaaatg tatcaaagag atagcaaggt attcagtttt agtaaacaag
      361 ataattgctc ctaaagtagc cccttgaatt ccgaggcagt aggcagccgc gattaggctg
      421 ttataataca tctgtggctt cactattata acagcctaat cgcggccgct cactgtcaac
      481 agcaatatac cttctcgagc cttctgttgg gttaacctga agaagtaatc ccagcaagtg
      541 tttccaagat gtgcaggcaa cgattctgta aagtactgaa gcctcattca acatagtata
      601 tgtgctgccg aagcgagcac ttaacaaggc ttgcggccgc ctacaggaac aggtggtggc
      661 ggccctcggc
//
