LOCUS       sequence-132482-         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression; in vitro transcription/translation (T7).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Grou CP, Pinto MP, Mendes AV, Domingues P, Azevedo JE
  TITLE     The de novo synthesis of ubiquitin: identification of 
            deubiquitinases acting on ubiquitin precursors.
  JOURNAL   Sci Rep. 2015 Aug 3;5:12836. doi: 10.1038/srep12836.
  PUBMED    26235645
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-08-3"; volume: "5:12836. doi"; pages: " 
            10.1038/srep12836"
COMMENT     Sequence Label: pET23a-HsUBB
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..250
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             23..250
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             251..478
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             251..478
                     /codon_start=1
                     /gene="Homo sapiens UBB"
                     /product="human ubiquitin B"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             479..706
                     /codon_start=1
                     /product="human ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     CDS             775..792
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 actttaagaa ggagatatac atatgcagat cttcgtgaaa acccttaccg gcaagaccat
       61 cacccttgag gtggagccca gtgacaccat cgaaaatgtg aaggccaaga tccaggataa
      121 ggaaggcatt ccccccgacc agcagaggct catctttgca ggcaagcagc tggaagatgg
      181 ccgtactctt tctgactaca acattcagaa ggagtcgacc ctgcacctgg tcctgcgtct
      241 gagaggtggt atgcagatct tcgtgaagac cctgaccggc aagaccatca ccctggaagt
      301 ggagcccagt gacaccatcg aaaatgtgaa ggccaagatc caggataaag aaggcatccc
      361 tcccgaccag cagaggctca tctttgcagg caagcagctg gaagatggcc gcactctttc
      421 tgactacaac atccagaagg agtcgaccct gcacctggtc ctgcgtctga gaggtggtat
      481 gcagatcttc gtgaagaccc tgaccggcaa gaccatcact ctggaggtgg agcccagtga
      541 caccatcgaa aatgtgaagg ccaagatcca agataaagaa ggcatccccc ccgaccagca
      601 gaggctcatc tttgcaggca agcagctgga agatggccgc actctttctg actacaacat
      661 ccagaaagag tcgaccctgc acctggtcct gcgcctgagg ggtggctgtt aattcttcag
      721 tcatggcagg atccgaattc gagctccgtc gacaagcttg cggccgcact cgagcaccac
      781 caccaccacc actgagatcc ggctgctaac aaagcccgaa aggaagctga
//
