LOCUS       sequence-132483-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dsRed and shRNA against vgat under the control of the CMV 
            promoter in eukaryotic cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Yu X, Ye Z, Houston CM, Zecharia AY, Ma Y, Zhang Z, Uygun DS, Parker
            S, Vyssotski AL, Yustos R, Franks NP, Brickley SG, Wisden W
  TITLE     Wakefulness Is Governed by GABA and Histamine Cotransmission.
  JOURNAL   Neuron. 2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003.
  PUBMED    26094607
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2015 Jun 17. pii: S0896-6273(15)00516-4. doi: 
            10.1016/j.neuron.2015.06.003."
COMMENT     Sequence Label: pPRIME-dsRed-shvgat
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(32..134)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     ncRNA           complement(288..414)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(442..456)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(484..605)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(717..740)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 cggtggtata tccagtgatt tttttctcca ttttagcttc cttagctcct gaaaatctcg
       61 acaactcaaa aaatacgccc ggtagtgatc ttatttcatt atggtgaaag ttggaacctc
      121 ttacgtgccg atcaacgtct cattttcgcc aaaagttggc ccagggcttc ccggtatcaa
      181 cagggacacc aggatttatt tattctgcga agtgatcttc cgtcacaggt atttattcgg
      241 tcgaacgcgt cgccgcgtgt ttaaacgcat tagtcttcca attgaaaaaa gtgatttaat
      301 ttataccatt ttaattcagc tttgtaaaaa tgtatcaaag agatagcaag gtattcagtt
      361 ttagtaaaca agataattgc tcctaaagta gccccttgaa ttccgaggca gtaggcaaac
      421 tcatcttgtg caatgtatct acatctgtgg cttcactaga tacattgcac aagatgagtg
      481 cgctcactgt caacagcaat ataccttctc gagccttctg ttgggttaac ctgaagaagt
      541 aatcccagca agtgtttcca agatgtgcag gcaacgattc tgtaaagtac tgaagcctca
      601 ttcaacatag tatatgtgct gccgaagcga gcacttaaca aggcttgcgg ccgcctacag
      661 gaacaggtgg tggcggccct cggcgcgctc gtactgctcc acgatggtgt agtcctcgtt
      721 gtgggaggtg atgtccagct tggagtccac gtagtagtag ccgggcagct gcacgggctt
      781 cttggccatg tagatggact tgaactccac c
//
