LOCUS       sequence-132486-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, RVG 
            peptide, 10 residue spacer, Lamp2b (exosomal transmembrane protein),
            3 residue spacer, HA tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Hung ME, Leonard JN
  TITLE     Stabilization of exosome-targeting peptides via engineered 
            glycosylation.
  JOURNAL   J Biol Chem. 2015 Mar 27;290(13):8166-72. doi: 
            10.1074/jbc.M114.621383. Epub 2015 Feb 5.
  PUBMED    25657008
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.621383"; journalName: "J Biol Chem"; date: 
            "2015-03-27- 27"; volume: "290"; issue: "13"; pages: "8166-72"
COMMENT     Sequence Label: pcDNA GNSTM-3-RVG-10-Lamp2b-HA
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agtgccgccg ccatggtgtg cttccgcctc ttcccggttc cgggctcagg
      121 gctcgttctg gtctgcctag tcctgggagc tgtgcggtct tatgcaggta actcgactat
      181 gggcagtgga tacaccattt ggatgcccga gaatccgaga ccagggacac cttgtgacat
      241 ttttaccaat agcagaggga agagagcatc caacgggggc agtggatctg gatccggtgg
      301 ctcgagtttg gaacttaatt tgacagattc agaaaatgcc acttgccttt atgcaaaatg
      361 gcagatgaat ttcacagttc gctatgaaac tacaaataaa acttataaaa ctgtaaccat
      421 ttcagaccat ggcactgtga catataatgg aagcatttgt ggggatgatc agaatggtcc
      481 caaaatagca gtgcagttcg gacctggctt ttcctggatt gcgaatttta ccaaggcagc
      541 atctacttat tcaattgaca gcgtctcatt ttcctacaac actggtgata acacaacatt
      601 tcctgatgct gaagataaag gaattcttac tgttgatgaa cttttggcca tcagaattcc
      661 attgaatgac ctttttagat gcaatagttt atcaactttg gaaaagaatg atgttgtcca
      721 acactactgg gatgttcttg tacaagcttt tgtccaaaat ggcacagtga gcacaaatga
      781 gttcctgtgt gataaagaca aaacttcaac agtggcaccc accatacaca ccactgtgcc
      841 atctcctact acaacaccta ctccaaagga aaaaccagaa gctggaacct attcagttaa
      901 taatggcaat gatacttgcc tgctggctac catgggg
//
