LOCUS       sequence-132490-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human full length SENP1 optimised for E. coli expression. 
            Suitable for in vitro transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP1
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             47..64
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..91
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata taccatgggc agcagccatc atcatcatca
       61 tcacagcagc ggcctggtgc cgcgcggcag ccatatggac gacattgccg accgtatgcg
      121 tatggatgcg ggcgaagtca ccctggttaa tcacaactca gtgttcaaaa cccacctgct
      181 gccgcaaacc ggcttcccgg aagatcagct gtccctgtca gaccagcaaa ttctgagctc
      241 tcgtcagggt catctggatc gcagctttac ctgttctacg cgtagtgcgg cctataatcc
      301 gtcctattac tcagataacc cgagttccga ctctttcctg ggcagtggtg atctgcgtac
      361 gtttggccaa tctgcgaatg gccagtggcg caacagtacc ccgtcatcga gctctagtct
      421 gcagaaatct cgtaattcgc gcagcctgta tctggaaacc cgcaaaacgt cctcaggcct
      481 gtctaatagt ttcgcaggta aaagtaacca tcactgtcat gtctccgctt acgaaaaatc
      541 atttccgatc aaaccggtgc cgtccccgtc atggagcggt agctgccgtc gctcgctgct
      601 gagcccgaag aaaacccagc gtcgccatgt gagcaccgca gaagaaacgg ttcaggaaga
      661 agaacgtgaa atttaccgcc aactgctgca gatggtgacg ggcaaacagt tcaccatcgc
      721 taaaccgacc acgcattttc cgctgcacct gtcccgttgt ctgtcgagct ctaaaaacac
      781 cctgaaagat tcactgttca aaaacggtaa ctcttgcgcg agtcagatta tcggcagtga
      841 cacgagttc
//
