LOCUS       sequence-132498-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human full length SENP2 optimised for E. coli expression. 
            Suitable for in vitro transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgtatcgc tggctggttc gtattctggg caccatcttc
      121 cgcttttgtg accgctctgt cccgccggca cgcgcactgc tgaaacgccg tcgctccgat
      181 tcaaccctgt tcagcacggt cgataccgac gaaattccgg cgaaacgtcc gcgcctggac
      241 tgctttatcc accaggttaa aaactccctg tataatgcgg cctcactgtt tggtttcccg
      301 tttcaactga ccacgaaacc gatggtgacg tctgcatgca acggcacccg taatgtcgct
      361 ccgtccggtg aagtgttttc aaacagctct agttgtgaac tgaccggctc gggtagctgg
      421 aacaatatgc tgaaactggg caacaaatcg ccgaatggta ttagcgatta cccgaaaatc
      481 cgtgtcaccg tgacgcgcga ccagccgcgt cgcgtgctgc cgtctttcgg ctttaccctg
      541 aacagtgaag gttgtaatcg tcgcccgggc ggtcgtcgcc actctaaagg taatccggaa
      601 tcctcactga tgtggaaacc gcaggaacaa gccgttacgg aaatgatttc cgaagaatca
      661 ggcaaaggtc tgcgtcgccc gcattgcacc gtggaagaag gcgttcagaa agaagaacgt
      721 gaaaaatatc gcaaactgct ggaacgtctg aaagaatctg gccatggtaa cagtgtttgt
      781 ccggtcacgt ccaattacca tagcagccag cgctcacaa
//
