LOCUS       sequence-132514-         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes human full length SENP5 optimised for E. coli expression. 
            Suitable for in vitro transcription/translation (T7)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Mendes AV, Grou CP, Azevedo JE, Pinto MP
  TITLE     Evaluation of the activity and substrate specificity of the human 
            SENP family of SUMO proteases.
  JOURNAL   Biochim Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020.
  PUBMED    26522917
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta. 2015 Oct 29. pii: S0167-4889(15)00373-0. doi: 
            10.1016/j.bbamcr.2015.10.020."
COMMENT     Sequence Label: pET28a-HsSENP5
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 actttaagaa ggagatatac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatgaaaaaa caacgtaaaa tcctgtggcg taaaggtatt
      121 catctggcat tctcggaaaa atggaacacg ggcttcggcg gcttcaaaaa attttatttc
      181 catcagcacc tgtgcattct gaaagcgaaa ctgggtcgtc cggtgacctg gaaccgtcaa
      241 ctgcgtcatt ttcagggtcg taaaaaagcc ctgcagattc aaaaaacgtg gatcaaagat
      301 gaaccgctgt gtgcgaaaac caaatttaac gtcgccaccc agaatgttag tacgctgagc
      361 tctaaagtga aacgcaaaga tgcaaaacat ttcatcagtt cctcaaaaac cctgctgcgt
      421 ctgcaggcag aaaaactgct gtcgagcgct aaaaacagcg atcacgaata ttgccgcgag
      481 aaaaacctgc tgaaagccgt tacggacttt ccgtccaact cagcactggg tcaagctaat
      541 ggtcatcgtc cgcgtaccga tccgcagccg agtgactttc cgatgaaatt caatggcgaa
      601 tcgcaaagcc cgggtgaatc cggcaccatt gtggttacgc tgaacaatca taaacgcaaa
      661 ggtttttgct acggctgctg tcagggtccg gaacatcacc gtaacggcgg tccgctgatt
      721 ccgaaaaaat tccagctgaa tcaacaccgt cgcatcaaac tgtcgccgct gatgatgtat
      781 gaaaaactga gcatgattcg ttttcgctac cgtatcctgc gctcgcagca tttccgtac
//
